课程概述
Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging.
A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses. We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content.
热门审阅
SP
Sep 22, 2019
Loved this course. Prof. Birtwistle is really into teaching and liked the way he has organized this course. Thanks!
FP
Jun 03, 2016
Excellent course with a wealth of information about system biology technologies
筛选依据：
26 - 系统生物学中的实验方法 的 28 个评论（共 28 个）
创建者 Pooja P
•Aug 15, 2016
The course was very interesting. It touched upon all currently employed techniques in good detail. The lab-based videos were the most interesting. However, the lectures were too long. It would be a brilliant course if the lectures were cut short and made to the point.
创建者 Rohaina T
•Sep 29, 2016
The questions based on the lectures are quite different and confusing
创建者 Alison P
•Apr 16, 2018
quiz 5 from week 6 does not work.
