课程信息
4.7
118 个评分
29 个审阅
100% 在线

100% 在线

立即开始,按照自己的计划学习。
可灵活调整截止日期

可灵活调整截止日期

根据您的日程表重置截止日期。
中级

中级

完成时间(小时)

完成时间大约为13 小时

建议:5 weeks, 2-3 hours/week...
可选语言

英语(English)

字幕:英语(English)

您将获得的技能

BiologySample PreparationSample CollectionCell Biology
100% 在线

100% 在线

立即开始,按照自己的计划学习。
可灵活调整截止日期

可灵活调整截止日期

根据您的日程表重置截止日期。
中级

中级

完成时间(小时)

完成时间大约为13 小时

建议:5 weeks, 2-3 hours/week...
可选语言

英语(English)

字幕:英语(English)

教学大纲 - 您将从这门课程中学到什么

1
完成时间(小时)
完成时间为 2 小时

Introduction to the Course and the Field of Extracellular Vesicles

In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles. ...
Reading
4 个视频 (总计 69 分钟), 1 个阅读材料, 1 个测验
Video4 个视频
Introduction to the Course11分钟
The Origin of EVs throughout the Phylogenetic Tree11分钟
History of Exosomes and Extracellular Vesicles32分钟
Reading1 个阅读材料
Syllabus10分钟
Quiz1 个练习
Introduction to Extracellular Vesicles18分钟
2
完成时间(小时)
完成时间为 3 小时

Biogenesis, Cargo and Uptake of Extracellular Vesicles

In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted....
Reading
6 个视频 (总计 142 分钟), 1 个测验
Video6 个视频
Mechanisms of Extracellular Vesicle Uptake - Part 122分钟
Mechanisms of Extracellular Vesicle Uptake - Part 223分钟
The Protein Content of Extracellular Vesicles23分钟
The RNA Content of Extracellular Vesicles35分钟
The Lipid Content of Extracellular Vesicles18分钟
Quiz1 个练习
Biogenesis, Cargo and Uptake of Extracellular Vesicles30分钟
3
完成时间(小时)
完成时间为 2 小时

Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles

In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids. ...
Reading
5 个视频 (总计 79 分钟), 1 个测验
Video5 个视频
Blood Plasma and Serum15分钟
Breast Milk8分钟
Urine25分钟
Cerebrospinal Fluid15分钟
Quiz1 个练习
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles36分钟
4
完成时间(小时)
完成时间为 2 小时

Methods for Isolating Extracellular Vesicles

This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits....
Reading
6 个视频 (总计 77 分钟), 1 个测验
Video6 个视频
Differential Ultracentrifugation - Part 216分钟
Density Gradient12分钟
Size Exclusion Chromatography14分钟
Kit-based Precipitation12分钟
Summary of Isolation Methods for Extracellular Vesicles6分钟
Quiz1 个练习
Methods for Isolating Extracellular Vesicles22分钟

讲师

Avatar

Cecilia Lasser

Researcher
Krefting Research Centre, University of Gothenburg

关于 University of California, Irvine

Since 1965, the University of California, Irvine has combined the strengths of a major research university with the bounty of an incomparable Southern California location. UCI’s unyielding commitment to rigorous academics, cutting-edge research, and leadership and character development makes the campus a driving force for innovation and discovery that serves our local, national and global communities in many ways....

常见问题

  • 注册以便获得证书后,您将有权访问所有视频、测验和编程作业(如果适用)。只有在您的班次开课之后,才可以提交和审阅同学互评作业。如果您选择在不购买的情况下浏览课程,可能无法访问某些作业。

  • 您购买证书后,将有权访问所有课程材料,包括评分作业。完成课程后,您的电子课程证书将添加到您的成就页中,您可以通过该页打印您的课程证书或将其添加到您的领英档案中。如果您只想阅读和查看课程内容,可以免费旁听课程。

还有其他问题吗?请访问 学生帮助中心