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学生对 丹麦技术大学 提供的 Whole genome sequencing of bacterial genomes - tools and applications 的评价和反馈

4.6
1,143 个评分
300 条评论

课程概述

This course will cover the topic of Whole genome sequencing (WGS) of bacterial genomes which is becoming more and more relevant for the medical sector. WGS technology and applications are high on international political agenda, as the classical methods are being replaced by WGS technology and therefore bioinformatic tools are extremely important for allowing the people working in this sector to be able to analyze the data and obtain results that can be interpreted and used for different purposes. The course will give the learners a basis to understand and be acquainted with WGS applications in surveillance of bacteria including species identification, typing and characterization of antimicrobial resistance and virulence traits as well as plasmid characterization. It will also give the opportunity to learners to learn about online tools and what they can be used for through demonstrations on how to use some of these tools and exercises to be solved by learners with use of freely available WGS analysis tools . By the end of this course you should be able to: 1. Describe the general Principles in typing of Bacteria 2. Give examples of the applications of Whole Genome Sequencing to Surveillance of bacterial pathogens and antimicrobial resistance 3. Apply genomic tools for sub-typing and surveillance 4. Define the concept of Next-Generation Sequencing and describe the sequencing data from NGS 5. Describe how to do de novo assembly from raw reads to contigs 6. Enumerate the methods behind the tools for species identification, MLST typing and resistance gene detection 7. Apply the tools for species identification, MLST typing and resistance gene detection in real cases of other bacterial and pathogen genomes. 8. Describe the methods behind the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing 9. Utilize the tools for Salmonella and E.coli typing, plasmid replicon detection and plasmid typing in real cases of other bacterial and pathogen genomes. 10. Explain the concept and be able to use the integrated bacterial analysis pipeline for batch analysis and typing of genomic data 11. Demonstrate how to construct phylogenetic tree based on SNPs 12. Apply the phylogenetic tool to construct phylogenetic trees and explain the relatedness of bacterial or pathogen strains 13. Describe how to create your own sequence database 14. Utilize the MyDbFinder tool to detect genetic markers of interest from whole genome sequencing...

热门审阅

AP

Jul 19, 2020

Thank you very much for this course through which i learnt basic handling of bioinformatics tools and concepts of WGS. This will help me a lot while pursuing my PhD research work in my field.

MB

Jun 9, 2019

Excellent course with a wealth of information about tools and applications of whole genome sequencing. Presented in a very lucid and interesting manner, very crispy and simplified course.

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201 - Whole genome sequencing of bacterial genomes - tools and applications 的 225 个评论(共 304 个)

创建者 Hajar F A

Apr 23, 2020

Really appreciate!

创建者 Dr A V

Jul 15, 2021

good intellectual

创建者 Ackshaya M S

Jan 21, 2022

very nice course

创建者 Md A

Jul 3, 2021

Excellent course

创建者 Shanmukha A P

Jun 8, 2020

Excellent course

创建者 davis e

May 23, 2022

amazing course

创建者 Khder h y

Nov 23, 2021

Thank you alot

创建者 Abdul F S

Jul 6, 2021

it was amazing

创建者 Christian A

Jun 6, 2020

Easy to follow

创建者 Bertram K

Aug 2, 2020

Great Course!

创建者 Mehdi G

Feb 26, 2022

Constructive

创建者 A A A E

Jan 8, 2019

applications

创建者 shuva s

Apr 9, 2018

Learnt a lot

创建者 Habeeb M

Jan 15, 2022

excellent

创建者 Bavani L

Jun 4, 2020

Nice good

创建者 Abhinandan C

Nov 16, 2017

Great One

创建者 Lelamekala V

Sep 28, 2021

Good

创建者 VASAVA M M

Jan 12, 2021

nice

创建者 Dr M

Sep 25, 2020

nice

创建者 Soumen P

Jun 29, 2021

创建者 BANSI S

Jul 31, 2020

.

创建者 Priya S

Nov 5, 2019

-

创建者 Signe A M

Nov 22, 2019

The course is an introduction to the Technical University of Denmark's (DTU) open access genomic tools only. Do not expect to learn about tools from other publishers.

Some of the questions for the quizzes were not covered by the video material, you either have to make educated guesses or find the information somewhere else. There is a long wait time for many of the tools, so remember to put in your e-mail address and do not expect to complete a quiz within the same day.

I preferred Rolf's lectures, they were short and well explained.

Overall I think it was a nice course for refreshing my memory but I don't believe this course is applicable outside university or similar research institutions (i.e. not so relevant if you need to integrate WGS at your workplace).

创建者 Robert G

Aug 19, 2017

This course provides a first hand view of whole genome sequencing (at the contig level anyway) as well as providing the concepts and tools for characterizing and typing bacteria and their associated plasmids. The course reviews the concepts and introduces the tools needed for determining species, type, and association between strains of bacteria. This course is directed toward microbiologists who wish to learn about using whole genome methods to type bacteria. However, anyone can learn the biology behind these methods

创建者 Maria S

Jan 5, 2018

Very good overview of the different CGE tools available for analysis of WGS bacterial genome data. Although it partly feels like pure advertisement of the tools and the videos describing usage of the different tools are very repetitive, overall the course gives a good insight. However, the instructors could put a bit more effort in answering the more complex quetions asked in the forum ins