[SOUND]. So that was several pathogen three major pathogens that we consider to be contagious. Now we want to look some environmental pathogens. So they are in the environment. So let's take a look at the first set. And I called it the first set because we collectively call these coliforms. They're environmental pathogens, they're all gram-negative and there's three species that which are very often found in terms of bovine mastitis. Eschericha coli, e-coli. Klebsiella oxytoca and klebsiella pneumoniae. Again, these are collectively called the coliform or coliform mastitis. They have a very similar response. And so I'm going to say they're not identical, obviously, because they're completely different species of bacterium, but the response of the cow is very, very similar. So that's why we collectively think of them as coliform. So coliform mastitis could be any of these three. Next slide, so, Coliform Mastitis. How do we think about this? All of these species inhabit the gastrointestinal tract, so, they're going to be in the manure, they're going to be in the environment. In the manure, in the bedding, polluted water, in the soil. So, anywhere that animal is potentially that these coliforms might be there. In during milking so when that street canal is open somehow those pathogens are getting in there. In addition to that we're told that when cows are milked especially with a machine that that street canal stays open, those sphincter muscles around the street canal. Or stay relaxed that the thing is open for roughly an hour after the cow is milked. So with a lot of times producers will do is, they'll feed cows right after, cows like to eat, they'll feed cows right after being milked. So the cow and most cows eat standing up so it gives them that time for those sphincter muscles to close that street canal down lessening the chance of getting some of these bacteria in the mammary gland. So as a way to manage the cows right after milking. It's highest, it likes hot, so it's highest in the summer, lowest in the winter months in terms of the, kind of the pathogen load. And the environment common in very early and late dry period in the peripartum, so around that time when the cow's calving, and then around the time or right after the time that she's dried off at the end of her lactation. It's most commonly when you'll have coliform mastitis occurring. Typically, it manifests itself as clinical mastitis, so as we think about we have an unusual number of cases of clinical mastitis. One of the first things we started thinking about is well, it might be environmental and it might be caused by the coliforms. Not a guarantee, but this is one way to kind of think and start to segregate out different types of mastitis and what's the cause of a particular problem in a herd. 50% of them were short durations, 10 days. They may actually cure themselves, so it is responsive to antibiotic therapy, but that may or may not. Again, if it's a fairly mild case of coliform mastitis, it might actually cure or clear itself out. So again, they're usually fairly short durations, so we don't normally find coliforms associated with chronic mastitis, which is over an extended period of time. It can progress to a peracute mastitis, and that's not at all unusual. But certainly if we see a lot of peracute mastitis, it could be caused by many different kind of bacteria. But a lot of times our first thought is, it's one of the coliforms causing the problem. About 1% of the cows prevalence. So it makes up to 30 to 40 % of the clinical cases. So if 2 to 4 % of the cows during their months period have clinical mastitis then a little less than half of those we can kind of assume will probably going to be caused by coliform mastitis. So that's a standard proportion that we might think of in a dairy herd. Another set of bacteria that we associate with environmental mastitis are called Environmental Streptococcal species or Environmental Strepts. So Strept uberis, Strept dysgalactiae, again, they inhabit gastrointestinal tracts, so they're going to be in the bedding. Especially kind of organic beddings such as straw we'd typically find them there. Common infections during the dry period so a lot of times they will be in the mammary gland. When you dry the cow off and of course you stop milking the cow so any bacteria in there going to cause a problem. Therefore dry cow antibiotic therapy is really very important for this particular bacteria as well as other kinds of bacteria. The prevalence increases in milk, wet milking. What we mean there is that washing the teat off, if the teat is still wet they could be out here, they could be on the bottom of the utter. And then again once that street canal is open they potentially can work their way in there. Dirty sponges, if you're using sponges we don't recommend that all that you use sponges anyway but dirty towels again washing one cow's teat using a towel or a sponge or cloth. Going to another cow you can potentially contaminate that cow's teat as well. These are usually a bit more mild, they typically don't go to pericyte as much as coliforms do, but certainly again, it's possible that they could. Again, clinical cases are usually mild, relatively speaking on average compared to again the coliform mastitis. So again another type of environmental pathogens that we associate with the environment again these environmental Streptococcus species. [SOUND]