课程信息
18,235

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初级

完成时间大约为13 小时

建议:12-18 hours videos and labs...

英语(English)

字幕:英语(English)

您将获得的技能

Genetic AnalysisBioinformatics AnalysisEvolutionComparative Genomics

100% 在线

立即开始,按照自己的计划学习。

可灵活调整截止日期

根据您的日程表重置截止日期。

初级

完成时间大约为13 小时

建议:12-18 hours videos and labs...

英语(English)

字幕:英语(English)

教学大纲 - 您将从这门课程中学到什么

1
完成时间为 3 小时

NCBI/Blast I

In this module we'll be exploring the amazing resources available at NCBI, the National Centre for Biotechnology Information, run by the National Library of Medicine in the USA. We'll also be doing a Blast search to find similar sequences in the enormous NR sequence database. We can use similar sequences to infer homology, which is the primary predictor of gene or protein function. ...
4 个视频 (总计 52 分钟), 4 个阅读材料, 1 个测验
4 个视频
Lecture25分钟
Lab Discussion24分钟
Summary
4 个阅读材料
Acknowledgements10分钟
Course Logistics10分钟
Lecture Materials10分钟
Lab 1 -- Exploring NCBI30分钟
1 个练习
Lab 1 Quiz6分钟
2
完成时间为 3 小时

Blast II/Comparative Genomics

In this module we'll continue exploring the incredible resources available at NCBI, the National Centre for Biotechnology Information. We will be performing several different kinds of Blast searches: BlastP, PSI-Blast, and Translated Blast. We can use similar sequences identified by such methods to infer homology, which is the primary predictor of gene or protein function. We'll also be comparing parts of the genomes of a couple of different species, to see how similar they are....
4 个视频 (总计 58 分钟), 2 个阅读材料, 1 个测验
4 个视频
Lecture32分钟
Lab Discussion23分钟
Summary
2 个阅读材料
Lecture Materials10分钟
Lab 2 -- Advanced Blast and Comparative Genomics30分钟
1 个练习
Lab 2 Quiz6分钟
3
完成时间为 2 小时

Multiple Sequence Alignments

In this module we'll be doing multiple sequence alignments with Clustal (as implemented in MEGA), DiAlign, and MAFFT. Multiple sequences alignments can tell you where in a sequence the conserved and variable regions are, which is important for understanding the biology of the sequences under investigation. It also has practical applications, such as being able to design PCR primers that will amplify sequences from a number of different species, for example....
4 个视频 (总计 42 分钟), 2 个阅读材料, 1 个测验
4 个视频
Lecture23分钟
Lab Discussion16分钟
Summary
2 个阅读材料
Lecture Materials10分钟
Lab 3 -- Multiple Sequence Alignment30分钟
1 个练习
Lab 3 Quiz6分钟
4
完成时间为 10 分钟

Review: NCBI/Blast I, Blast II/Comparative Genetics, and Multiple Sequence Alignments

...
1 个测验
1 个练习
Quiz: Modules 1-310分钟
5
完成时间为 2 小时

Phylogenetics

In this module we'll be using the multiple sequence alignments we generated last lab to do some phylogenetic analyses with both neighbour-joining and maximum likelihood methods. The tree-like structure generated by such analyses tells us how closely sequences are related one to another, and suggests when in evolutionary time a speciation or gene duplication event occurred....
4 个视频 (总计 36 分钟), 2 个阅读材料, 1 个测验
4 个视频
Lecture25分钟
Lab Discussion9分钟
Summary
2 个阅读材料
Lecture Materials10分钟
Lab 4 -- Phylogenetics30分钟
1 个练习
Lab 4 Quiz6分钟
6
完成时间为 2 小时

Selection Analysis

In this module we'll take a set of orthologous sequences from bacteria and use DataMonkey to analyze them for the presence of certain sites under positive, negative or neutral selection. Such an analysis can help understand the biology of a set of protein coding sequences by identifying residues that might be important for biological function (those residues under negative selection) or those that might be involved in response to external influences, such as drugs, pathogens or other factors (residues under positive selection)....
4 个视频 (总计 38 分钟), 2 个阅读材料, 1 个测验
4 个视频
Lecture23分钟
Lab Discussion10分钟
Summary1分钟
2 个阅读材料
Lecture Materials10分钟
Lab 5 -- Selection Analysis30分钟
1 个练习
Lab 5 Quiz6分钟
7
完成时间为 3 小时

'Next Gen' Sequence Analysis (RNA-Seq) / Metagenomics

In this module we'll explore some of the data that have been generated as a result of the rapid decrease in the cost of sequencing DNA. We'll be exploring a couple of RNA-Seq data sets that can tell us where any given gene is expressed, and also how that gene might be alternatively spliced. We'll also be looking at a couple of metagenome data sets that can tell us about the kinds of species (especially microbial species that might otherwise be hard to culture) that are in a given environmental niche....
4 个视频 (总计 51 分钟), 2 个阅读材料, 1 个测验
4 个视频
Lecture24分钟
Lab Discussion20分钟
Summary4分钟
2 个阅读材料
Lecture Materials10分钟
Lab 6 -- Next Generation Sequencing Applications: RNA-Seq and Metagenomics30分钟
1 个练习
Lab 6 Quiz6分钟
8
完成时间为 1 小时

Review: Phylogenetics, Selection Analysis, and 'Next Gen' Sequence Analysis (RNA-seq)/Metagenomics + Final Assignment

...
1 个阅读材料, 2 个测验
1 个阅读材料
Final Assignment Instructions10分钟
2 个练习
Review: Modules 5-710分钟
Final Assignment10分钟
4.7
158 个审阅Chevron Right

21%

完成这些课程后已开始新的职业生涯

29%

通过此课程获得实实在在的工作福利

20%

加薪或升职

热门审阅

创建者 EDApr 27th 2017

Great course. All lectures provide a biological context for the tools you learn in the labs. The labs themselves provide a great introduction to the many tools available for bioinformatic analysis.

创建者 FJFeb 6th 2017

The videos were very clear on how to use various bioinformatic tools. I found no difficulty in understanding them. Really enjoyed the course. Looking forward to the next part of this course!

讲师

Avatar

Nicholas James Provart

Professor
Cell & Systems Biology

关于 多伦多大学

Established in 1827, the University of Toronto is one of the world’s leading universities, renowned for its excellence in teaching, research, innovation and entrepreneurship, as well as its impact on economic prosperity and social well-being around the globe. ...

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