课程信息
4.7
619 ratings
140 reviews
Large-scale biology projects such as the sequencing of the human genome and gene expression surveys using RNA-seq, microarrays and other technologies have created a wealth of data for biologists. However, the challenge facing scientists is analyzing and even accessing these data to extract useful information pertaining to the system being studied. This course focuses on employing existing bioinformatic resources – mainly web-based programs and databases – to access the wealth of data to answer questions relevant to the average biologist, and is highly hands-on. Topics covered include multiple sequence alignments, phylogenetics, gene expression data analysis, and protein interaction networks, in two separate parts. The first part, Bioinformatic Methods I (this one), deals with databases, Blast, multiple sequence alignments, phylogenetics, selection analysis and metagenomics. This, the second part, Bioinformatic Methods II, covers motif searching, protein-protein interactions, structural bioinformatics, gene expression data analysis, and cis-element predictions. This pair of courses is useful to any student considering graduate school in the biological sciences, as well as students considering molecular medicine. Both provide an overview of the many different bioinformatic tools that are out there. These courses are based on one taught at the University of Toronto to upper-level undergraduates who have some understanding of basic molecular biology. If you're not familiar with this, something like https://learn.saylor.org/course/bio101 might be helpful. No programming is required for this course....
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建议:12-18 hours videos and labs

完成时间大约为12 小时
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您将获得的技能

Genetic AnalysisBioinformatics AnalysisEvolutionComparative Genomics
Globe

100% 在线课程

立即开始,按照自己的计划学习。
Calendar

可灵活调整截止日期

根据您的日程表重置截止日期。
Clock

建议:12-18 hours videos and labs

完成时间大约为12 小时
Comment Dots

English

字幕:English

教学大纲 - 您将从这门课程中学到什么

1

章节
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完成时间为 3 小时

NCBI/Blast I

In this module we'll be exploring the amazing resources available at NCBI, the National Centre for Biotechnology Information, run by the National Library of Medicine in the USA. We'll also be doing a Blast search to find similar sequences in the enormous NR sequence database. We can use similar sequences to infer homology, which is the primary predictor of gene or protein function. ...
Reading
4 个视频(共 52 分钟), 4 个阅读材料, 1 个测验
Video4 个视频
Lecture25分钟
Lab Discussion24分钟
Summary分钟
Reading4 个阅读材料
Acknowledgements10分钟
Course Logistics10分钟
Lecture Materials10分钟
Lab 1 -- Exploring NCBI30分钟
Quiz1 个练习
Lab 1 Quiz6分钟

2

章节
Clock
完成时间为 3 小时

Blast II/Comparative Genomics

In this module we'll continue exploring the incredible resources available at NCBI, the National Centre for Biotechnology Information. We will be performing several different kinds of Blast searches: BlastP, PSI-Blast, and Translated Blast. We can use similar sequences identified by such methods to infer homology, which is the primary predictor of gene or protein function. We'll also be comparing parts of the genomes of a couple of different species, to see how similar they are....
Reading
4 个视频(共 58 分钟), 2 个阅读材料, 1 个测验
Video4 个视频
Lecture32分钟
Lab Discussion23分钟
Summary分钟
Reading2 个阅读材料
Lecture Materials10分钟
Lab 2 -- Advanced Blast and Comparative Genomics30分钟
Quiz1 个练习
Lab 2 Quiz6分钟

3

章节
Clock
完成时间为 2 小时

Multiple Sequence Alignments

In this module we'll be doing multiple sequence alignments with Clustal (as implemented in MEGA), DiAlign, and MAFFT. Multiple sequences alignments can tell you where in a sequence the conserved and variable regions are, which is important for understanding the biology of the sequences under investigation. It also has practical applications, such as being able to design PCR primers that will amplify sequences from a number of different species, for example....
Reading
4 个视频(共 42 分钟), 2 个阅读材料, 1 个测验
Video4 个视频
Lecture23分钟
Lab Discussion16分钟
Summary分钟
Reading2 个阅读材料
Lecture Materials10分钟
Lab 3 -- Multiple Sequence Alignment30分钟
Quiz1 个练习
Lab 3 Quiz6分钟

4

章节
Clock
完成时间为 10 分钟

Review: NCBI/Blast I, Blast II/Comparative Genetics, and Multiple Sequence Alignments

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Reading
1 个测验
Quiz1 个练习
Quiz: Modules 1-310分钟
4.7
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18%

完成这些课程后已开始新的职业生涯
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通过此课程获得实实在在的工作福利
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热门审阅

创建者 EDApr 27th 2017

Great course. All lectures provide a biological context for the tools you learn in the labs. The labs themselves provide a great introduction to the many tools available for bioinformatic analysis.

创建者 FJFeb 6th 2017

The videos were very clear on how to use various bioinformatic tools. I found no difficulty in understanding them. Really enjoyed the course. Looking forward to the next part of this course!

讲师

Nicholas James Provart

Professor
Cell & Systems Biology

关于 University of Toronto

Established in 1827, the University of Toronto has one of the strongest research and teaching faculties in North America, presenting top students at all levels with an intellectual environment unmatched in depth and breadth on any other Canadian campus. ...

常见问题

  • Once you enroll for a Certificate, you’ll have access to all videos, quizzes, and programming assignments (if applicable). Peer review assignments can only be submitted and reviewed once your session has begun. If you choose to explore the course without purchasing, you may not be able to access certain assignments.

  • When you purchase a Certificate you get access to all course materials, including graded assignments. Upon completing the course, your electronic Certificate will be added to your Accomplishments page - from there, you can print your Certificate or add it to your LinkedIn profile. If you only want to read and view the course content, you can audit the course for free.

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