This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques.
Basics of Extracellular Vesicles
加州大学尔湾分校课程信息
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中级
完成时间大约为10 小时
英语（English）
字幕：英语（English）
您将获得的技能
BiologySample PreparationSample CollectionCell Biology
学生职业成果
50%
完成这些课程后已开始新的职业生涯
可分享的证书
完成后获得证书
100% 在线
立即开始，按照自己的计划学习。
可灵活调整截止日期
根据您的日程表重置截止日期。
中级
完成时间大约为10 小时
英语（English）
字幕：英语（English）
提供方
加州大学尔湾分校
Since 1965, the University of California, Irvine has combined the strengths of a major research university with the bounty of an incomparable Southern California location. UCI’s unyielding commitment to rigorous academics, cutting-edge research, and leadership and character development makes the campus a driving force for innovation and discovery that serves our local, national and global communities in many ways.
教学大纲 - 您将从这门课程中学到什么
完成时间为 2 小时
Introduction to the Course and the Field of Extracellular Vesicles
In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles.
完成时间为 2 小时
4 个视频 （总计 69 分钟）, 1 个阅读材料, 1 个测验
4 个视频
Introduction to the Course11分钟
The Origin of EVs throughout the Phylogenetic Tree11分钟
History of Exosomes and Extracellular Vesicles32分钟
1 个阅读材料
Syllabus10分钟
1 个练习
Introduction to Extracellular Vesicles18分钟
完成时间为 3 小时
Biogenesis, Cargo and Uptake of Extracellular Vesicles
In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted.
完成时间为 3 小时
6 个视频 （总计 142 分钟）
6 个视频
Mechanisms of Extracellular Vesicle Uptake - Part 122分钟
Mechanisms of Extracellular Vesicle Uptake - Part 223分钟
The Protein Content of Extracellular Vesicles23分钟
The RNA Content of Extracellular Vesicles35分钟
The Lipid Content of Extracellular Vesicles18分钟
1 个练习
Biogenesis, Cargo and Uptake of Extracellular Vesicles30分钟
完成时间为 2 小时
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles
In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids.
完成时间为 2 小时
5 个视频 （总计 79 分钟）
1 个练习
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles36分钟
完成时间为 2 小时
Methods for Isolating Extracellular Vesicles
This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits.
完成时间为 2 小时
6 个视频 （总计 77 分钟）
6 个视频
Differential Ultracentrifugation - Part 216分钟
Density Gradient12分钟
Size Exclusion Chromatography14分钟
Kit-based Precipitation12分钟
Summary of Isolation Methods for Extracellular Vesicles6分钟
1 个练习
Methods for Isolating Extracellular Vesicles22分钟
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