课程信息
4.8
343 ratings
91 reviews
While the human genome sequence has transformed our understanding of human biology, it isn’t just the sequence of your DNA that matters, but also how you use it! How are some genes activated and others are silenced? How is this controlled? The answer is epigenetics. Epigenetics has been a hot topic for research over the past decade as it has become clear that aberrant epigenetic control contributes to disease (particularly to cancer). Epigenetic alterations are heritable through cell division, and in some instances are able to behave similarly to mutations in terms of their stability. Importantly, unlike genetic mutations, epigenetic modifications are reversible and therefore have the potential to be manipulated therapeutically. It has also become clear in recent years that epigenetic modifications are sensitive to the environment (for example diet), which has sparked a large amount of public debate and research. This course will give an introduction to the fundamentals of epigenetic control. We will examine epigenetic phenomena that are manifestations of epigenetic control in several organisms, with a focus on mammals. We will examine the interplay between epigenetic control and the environment and finally the role of aberrant epigenetic control in disease. All necessary information will be covered in the lectures, and recommended and required readings will be provided. There are no additional required texts for this course. For those interested, additional information can be obtained in the following textbook. Epigenetics. Allis, Jenuwein, Reinberg and Caparros. Cold Spring Harbour Laboratory Press. ISBN-13: 978-0879697242 | Edition: 1...
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建议:7 weeks of study, 6-8 hours/week

完成时间大约为29 小时
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您将获得的技能

Cancer EpigeneticsDna MethylationCancerMolecular Biology
Globe

100% 在线课程

立即开始,按照自己的计划学习。
Calendar

可灵活调整截止日期

根据您的日程表重置截止日期。
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建议:7 weeks of study, 6-8 hours/week

完成时间大约为29 小时
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English

字幕:English

教学大纲 - 您将从这门课程中学到什么

1

章节
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完成时间为 2 小时

Week 1 - Introduction to Epigenetic Control

An introduction to and definition of epigenetic control of gene expression, and its importance in normal development. We will learn what chromatin is, and how its composition and packaging can alter gene expression. We’ll also discuss the best-characterised epigenetic modification, DNA methylation, and how it is not only implicated in regulating gene expression, but also in maintaining genome stability. ...
Reading
7 个视频(共 65 分钟), 5 个阅读材料, 1 个测验
Video7 个视频
1.1 Introduction to the concepts of epigenetic control10分钟
1.2 Mitotic heritability of epigenetic marks10分钟
1.3 Chromatin and the nucleosome6分钟
1.4 Chromatin compaction - heterochromatin versus euchromatin9分钟
1.5 DNA methylation at CpG islands11分钟
1.6 DNA methylation at intergenic regions and repetitive elements15分钟
Reading5 个阅读材料
Course syllabus10分钟
Teaching team10分钟
Start of course survey10分钟
Assessment and grading policy10分钟
Week 1 and 2 resources10分钟
Quiz1 个练习
Week 1 quiz - contributes 8% towards your final grade20分钟

2

章节
Clock
完成时间为 2 小时

Week 2 - Epigenetic Modifications and Organisation of the Nucleus

We’ll discuss the molecular mechanisms for regulating gene expression in some detail, from how the DNA is packaged at a local level, right up to how the chromatin is positioned within the nucleus. We’ll learn about the chromatin modifications implicated in gene silencing and activation, the role of non-coding RNA, and higher order chromatin structures. This week will provide you with a good understanding of the basic mechanisms that will help you understand the processes we discuss throughout the rest of the course. ...
Reading
9 个视频(共 90 分钟), 1 个阅读材料, 1 个测验
Video9 个视频
2.2 Histone acetylation and histone methylation12分钟
2.3 Chromatin remodelling12分钟
2.4 Histone variants8分钟
2.5 Noncoding RNAs - microRNAs6分钟
2.6 Noncoding RNAs - piRNAs9分钟
2.7 Noncoding RNAs - long noncoding RNAs introduction10分钟
2.8 Long noncoding RNAs Xist and HOTAIR7分钟
2.9 3D organisation of the nucleus and summary of epigenetic marks17分钟
Reading1 个阅读材料
Week 1 and 2 resources10分钟
Quiz1 个练习
Week 2 quiz - contributes 8% towards your final grade20分钟

3

章节
Clock
完成时间为 3 小时

Week 3 - Dosage Compensation

X chromosome inactivation is a really well-characterised epigenetic process that is now used as a model system to study epigenetic processes that are relevant more broadly. This is because it uses many epigenetic mechanisms, at many levels, to achieve really stable silencing of a whole chromosome. We’ll learn about this process and how it occurs in a mouse in great detail, which will greatly add to the mechanistic understanding gained in week two. We will then briefly discuss alternate mechanisms for dosage compensation that occur in other organisms. ...
Reading
12 个视频(共 113 分钟), 1 个阅读材料, 1 个测验
Video12 个视频
3.2 Timing of random and imprinted X chromosome inactivation8分钟
3.3 Stages of X inactivation - counting and control of Xist expression13分钟
3.4 Control of Xist expression by pluripotency factors6分钟
3.5 Stages of X inactivation - choice of which X to inactivate9分钟
3.6 Stages of X inactivation - initiation and spreading of silencing11分钟
3.7 Stages of X inactivation - establishment of silencing6分钟
3.8 Stages of X inactivation - maintenance of silencing e.g. Dnmt18分钟
3.9 Stages of X inactivation - maintenance of silencing e.g. Smchd18分钟
3.10 X chromosome inactivation summary10分钟
3.11 Dosage compensation in flies and worms, compared with mammals8分钟
3.12 Lessons from the fly - position effect variegation and screening for epigenetic modifiers10分钟
Reading1 个阅读材料
Week 3 resources (including required readings)10分钟
Quiz1 个练习
Week 3 quiz - contributes 12% towards your final grade30分钟

4

章节
Clock
完成时间为 2 小时

Week 4 - Genomic Imprinting and Epigenetic Reprogramming

We’ll learn about the two important periods during development for the erasure and resetting of the epigenome. There are two well-characterised features that are treated differently during epigenetic reprogramming; imprinted genes and repeats. We’ll learn about mechanisms for genomic imprinting, and study three examples in more depth....
Reading
6 个视频(共 68 分钟), 1 个阅读材料, 1 个测验
Video6 个视频
4.2 Epigenetic reprogramming of imprinted genes and repetitive elements11分钟
4.3 Location of imprinted genes in the genome and bisulfite sequencing10分钟
4.4 Kcnq1 and H19/Igf2 ICR mechanisms of action and Beckwith Weidemann syndrome17分钟
4.5 Snrpn ICR mechanism, Prader Willi and Angelman syndromes13分钟
4.6 Summary of epigenetic reprogramming and imprinting6分钟
Reading1 个阅读材料
Week 4 resources (including required reading)10分钟
Quiz1 个练习
Week 4 quiz - contributes 12% towards your final grade30分钟
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创建者 BMAug 14th 2017

One of the best organized and implemented courses I have taken so far. For someone who had barely any experience with epigenetics, I feel this gave me a proper introduction to the field.

创建者 HHDec 18th 2016

A quintessential course for Genetics students all over the world. Very nicely prepared and elucidated by Dr. Marnie Blewitt .\n\nMany many thanks to Dr. Marnie Blewitt & Coursera team.

讲师

Dr. Marnie Blewitt

Head, Molecular Medicine Laboratory
Walter and Eliza Hall Institute of Medical Research

关于 The University of Melbourne

The University of Melbourne is an internationally recognised research intensive University with a strong tradition of excellence in teaching, research, and community engagement. Established in 1853, it is Australia's second oldest University....

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