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Experimental Methods in Systems Biology, Icahn School of Medicine at Mount Sinai

4.6
148 个评分
23 个审阅

课程信息

Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging. A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses. We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content....

热门审阅

创建者 FP

Jun 03, 2016

Excellent course with a wealth of information about system biology technologies

创建者 EM

Dec 04, 2015

Great course,\n\nRecommended if you are interest in new technologies

筛选依据:

23 个审阅

创建者 Pablo Llevenes

Nov 21, 2018

Really good course, even though the test were so unfair with the multiple choice questions. Why not to give any simple 0.2 or 0.25 if you get one answer out of 5? Why do I have to select all ofthem correctly if not I do not receive any point? Very unfair, 4 stars because of this. the rest of it is perfect even with lab based videos.

创建者 Seema Parte

Oct 02, 2018

Way to go..enjoyed learning

创建者 Alison Paquette

Apr 16, 2018

quiz 5 from week 6 does not work.

创建者 Mac Dang Giang

Jan 24, 2018

it was so useful

创建者 李俊宏

Dec 07, 2017

The course is mainly focused on description of the technologies used in system biology. Mechanisms of these experiments may not be very clear which makes this course seems like an introduction to the experiments. Also the quizzes are too long with enormous words, sometimes it's like a game of finding differences. I hope the quizzes be more focused on principals and calculation, which will make it more clearer.

Finally, the teacher and all staff have made a lot of efforts to build this nice course! Thank you so much!

创建者 Mai Le

Sep 30, 2017

The course provides up to date understanding of new technologies. The examples are carefully analyzed and explained. The course is very helpful for those who want to follow lab-based research. However, learners must be aware that the amount of knowledge you take in for each lesson is very large, you might need to watch the lesson over and over again to actually understand deeply.

创建者 Jiho Choi

Mar 09, 2017

Good lecture to know experimental method in lab

创建者 Weidong Xu

Dec 04, 2016

good.

创建者 Mrinal Vashisth

Oct 22, 2016

Loved it! :D

创建者 Rohaina Tanveer

Sep 29, 2016

The questions based on the lectures are quite different and confusing