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学生对 加州大学尔湾分校 提供的 Basics of Extracellular Vesicles 的评价和反馈

4.8
309 个评分
85 条评论

课程概述

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

热门审阅

MH
Jan 10, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD
May 16, 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

筛选依据:

51 - Basics of Extracellular Vesicles 的 75 个评论(共 82 个)

创建者 Laura M M E

May 4, 2020

Excelente curso, expositores con amplio conocimiento del tema

创建者 Stephanie B

Oct 5, 2019

Good basic overview for someone starting in their studies

创建者 Wenhao L

Apr 26, 2017

Very wonderful courses! Better than reading 100 papers!

创建者 Mohammad R

Feb 5, 2017

This course gives an excellent overview of the field!

创建者 Nahla I S H

Apr 22, 2020

A very good course, really it was very informative.

创建者 pbmansfield

Mar 6, 2017

Well put together and very informative. Great job!

创建者 Dr.� S R S A P -

Jun 26, 2020

very helpful and nice videos and the contents too

创建者 Ramiro R d l C

Nov 4, 2020

Great! Very informative and I learned a lot.

创建者 Emilio E S

Jul 26, 2021

really good course. I learn a lot about EVs

创建者 Regina C

Dec 29, 2020

Very interesting and simple to understand

创建者 Olivia C

Aug 6, 2020

very useful course, I really enjoy it!

创建者 Yingchao M

Apr 9, 2020

Very good for beginners.

创建者 David H

Sep 1, 2020

excellent course

创建者 Abner M N

Nov 21, 2019

Amazing course!

创建者 Dr T S R

Sep 17, 2020

Super Course

创建者 Adel D

Nov 3, 2016

very good!

创建者 CAROLINA T P

Jul 30, 2021

Excellent

创建者 ganesha p

Sep 16, 2020

Excellent

创建者 Lee-Ann M C

Jul 9, 2021

Usefull

创建者 Kaio M R

Oct 9, 2017

ÓTIMO

创建者 Emayamsavitha

Apr 27, 2020

Gud

创建者 PRIYASHREE N

Jul 3, 2020

The way the course was introduced and was taught is really amazing. There were few issues in understanding the audio and there was problem with subtitles as well but still it was a good and amazing experience to meet and listen to researchers from various parts of world. Though it is a blooming field, there was a lot of information in this course. And I gained lot of information from this course. Thanks for every single researcher and professor who are involved in this course. It was a great pleasure to meet and hear your lectures.

创建者 Arjun J

Sep 7, 2021

Definitely got to learn quite a bit of possibly useful stuff (I'm very new to this) during the course. The quality of lectures varied on a spectrum from average to great, depending on the instructor for each topic. While some of them literally just read off the slides they prepared, others delivered the info with evident passion, which kept you attentive and interested. It's a mixed bag, but don't let that stop you - overall, the course experience was not bad.

创建者 Mainak B

Nov 2, 2019

Excellent content for learning about basic of extracellular vesicles beginners. One thing I would like to point out is it would have been great if the course materials are made available as a pdf version.

创建者 Enrique V O

May 11, 2017

It is good much info about vesicles that you can't get from the books and techniques to study them.