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学生对 加州大学尔湾分校 提供的 Basics of Extracellular Vesicles 的评价和反馈

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241 个评分
61 条评论

课程概述

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

热门审阅

MH

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

DD

May 17, 2020

I found Ia very professional way to introduce everyone to the amazing world of EV. Professor show elegantly their knowledge. The course fulfils my expectations. Congratulations! Nice works

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51 - Basics of Extracellular Vesicles 的 59 个评论(共 59 个)

创建者 Emayamsavitha

Apr 27, 2020

Gud

创建者 PRIYASHREE N

Jul 03, 2020

The way the course was introduced and was taught is really amazing. There were few issues in understanding the audio and there was problem with subtitles as well but still it was a good and amazing experience to meet and listen to researchers from various parts of world. Though it is a blooming field, there was a lot of information in this course. And I gained lot of information from this course. Thanks for every single researcher and professor who are involved in this course. It was a great pleasure to meet and hear your lectures.

创建者 Mainak B

Nov 02, 2019

Excellent content for learning about basic of extracellular vesicles beginners. One thing I would like to point out is it would have been great if the course materials are made available as a pdf version.

创建者 Enrique V O

May 11, 2017

It is good much info about vesicles that you can't get from the books and techniques to study them.

创建者 Shalmali P

Mar 03, 2018

Very nice nd inormative presentations. But few words in many presentations are not audible.

创建者 Marine H

Aug 11, 2020

The last two modules were extremely helpful and a very good opener to this topic.

创建者 David d V L

Mar 29, 2018

Excellents classes I have learned a lot of procotols about EVs

创建者 Xiaokun L

Jul 20, 2017

nice

创建者 lcy9086

Apr 01, 2018

I am sorry to say that many parts of the lecture provided by the Korean professor is inaudible......SO you can only learn from his slides :(